Relationship Between Traditional Medical Formulae and Syndrome Based Pharmacological Study of Xiayuxue Decoction against Porcine Serum Induced Liver Fibrosis in Rats

Objective:To study relationship between traditional medical formulae and syndrome of Xiayuxue Decotion against liver fibrosis in rats induced by porcine serum.Methods:Liver fibrosis in rats was induced by intraperitoneal injection of porcine serum.10 rats(4 in normal group and 6 in model group)were sacrificed after 8 w of modelling.The rest rats were then devided into normal group,model group,Xiayuxue Decoction group,Yinchenhao Decoction group,Huangqi Dectoction group,Erzhi Pill Group and Xiaochaihu Decoction Group.Porcine serum was continuous given at the same time of administration.Rats were sacrificed and sample was obtained at the end of 12 w of modeling.ALT,AST,ALP and GGT activity,and Alb,TP and TBil content in rats were determined.Hyp content in rat liver tissue was assayed with hydrochloric acid hydrolyzing method,and histological changes with HE staining and collagen deposition with sirus red staining.Results:ALT and AST levels increased significantly(P0.05),Alb and TP content decrease markedly(P0.05).For the increased ALP,GGT and TBil levels there are no.Compared with model rats the improved liver function of rats in treatment groups is found especially in Xiayuxue Decoction.Compared with normal group,Hyp content of liver tissue in modeling rats increased significantly(P0.01).Compared with model group,liver tissue Hyp content in rats of Xiayuxue Decoction group decreases significantly(P0.05),Hyp content in Yinchenhao Decoction group also decreases to some extent without statistical significance(P0.05).Conclusion:Xiayuxue Decoction effectively inhibits liver fibrosis in rats induced by porcine serum,which indicated that liver fibrosis induced by porcine serum in rats might be blood stasis congesting vessels.