Protective Effect of Ronggan Mixture on the Mice Model with the Concanavalin A-Induced Acute Immunological Liver Injury

Objective To investigate the protective effect and mechanism of Ronggan Mixture(Herbal Decoction for liver diseases)on the mice model with concanavalin A-induced acute immunological liver injury.Methods Totally 60 HBV transgenic mice were randomized into model group,control group,Ronggan Mixture group,Yinchenhao Decoction group,Yinchen group,and Bifendate group,with 10 in each.The acute immunological liver injury models were established by the tail vein injection of concanavalin A.Fourteen days before modeling,the model group and control group were given normal saline by gavage,while the other groups were treated with Ronggan Mixture,Yinchenhao Decoction(Oriental Wormwood Decoction),Yinchen(Virgate Wormwood Herb),and Bifendate respectively by gavage everyday.One hour after the last intervention,the control group was given phosphate buffer solution(PBS)by the tail vein injection,and the others were given concanavalin A by the tail vein injection to establish models.Eight hours after modeling,the mice were sacrificed,the serum and tissue samples were collected to detect:alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(TBiL),hepatitis B virus quantity(HBV DNA),hepatitis B surface antigen(HBsAg),superoxide dismutase(SOD),and malondialdehyde(MDA).Results Compared with the model group and the others,Ronggan Mixture could decrease the level of ALT,AST,and TBiL(P0.05 or P0.01).Compared with the Bifendate group,Yinchen group,and Yinchenhao Decoction group,Ronggan Mixture could decrease the HBV DNA and the MDA of liver tissues,and increase SOD level of liver tissues.Conclusion Ronggan Mixture is of advantage in decreasing the enzymes as compared with Yinchen,Yinchenhao Decoction and Bifendate.The liver protective effect is achieved through inhibiting HBV DNA to reduce the injury of the lipid peroxidation of hepatocyte membrane.