DNA Damage of Human Umbilical Vein Endothelial Cells Caused by Formaldehyde and H_2O_2 with Comet Assay and K-SDS Assay

BACKGROUND AIM: To investigate DNA damage of human umbilical vein endothelial cells after treated with formaldehyde, H2O2, formaldehyde and H2O2. MATERIAL AND METHODS: ①Comet assay was employed to assess DNA damage of human umbilical vein endothelial cells treated with either various concentrations (0, 5, 10, 25, 50, 100μmol/L) of formaldehyde, H2O2, formaldehyde and H2O2 for 20 min or 25μmol/L formaldehyde, H2O2, formaldehyde and H2O2 for different time(0, 10, 20, 30 min) to quantify the DNA damage. ②K-SDS was employed to evaluate DNA-Protein cross-link(DPC) of endothelial cells treated with either various concentrations of formaldehyde, formaldehyde and H202(0, 10, 50, 100, 500, 1 000, 2 000 μmol/L) for 1.5 h or 50 μmol/L and 100 mol/L formaldehyde, formaldehyde and H2O2 for different time(0,0.5,1,1. 5,2,4 h) to quantify the DPC. RESULTS: DNA breakage caused by 5, 10, 25μmol/L formaldehyde was significantly different from control, and might be increased by H2O2. Tail moment was time-dependent when treated with 25μmol/L formaldehyde. The formation of DPC increased(P 0.05)after treated with various concentrations of formaldehyde for 1.5 h. CONCLUSION:①Formaldehyde can cause DNA breakage( 25μmol/L) and DPC( 500 μmol/L), both were concentration and time-dependent.②H2O2 can cause DNA breakage, which was concentration and time-dependent.