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2006•Research and Practice on Chinese MedicinesRequires access

Determination of Baicalin in Changyijie Granules by HPLC

Qin Xia-chan

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Abstract

Objective To determine the content of baicalin in Changyijie granules.Methods HPLC was used with Phenomenex-C_(18)column(4.6 mm×250 mm),the mobile phase was methanol-0.1%H_3PO_4(49∶51),detection wavelength was at 280 nm.Results The standard curves of baicalin was the linear over the rang of 0.01～0.393 mg·mL~(-1) and the recovery was 96.02%(RSD=2.35%).Conclusion The method is accurate,sensitive,reproductive and can be used for quality control of Changyijie granules.

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What this paper is about

Objective To determine the content of baicalin in Changyijie granules.Methods HPLC was used with Phenomenex-C_(18)column(4.6 mm×250 mm),the mobile phase was methanol-0.1%H_3PO_4(49∶51),detection wavelength was at 280 nm.Results The standard curves of baicalin was the linear over the rang of 0.01～0.393 mg·mL~(-1) and the recovery was 96.02%(RSD=2.35%).Conclusion The method is accurate,sensitive,reproductive and can be used for quality control of Changyijie granules.

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Available abstract

Objective To determine the content of baicalin in Changyijie granules.Methods HPLC was used with Phenomenex-C_(18)column(4.6 mm×250 mm),the mobile phase was methanol-0.1%H_3PO_4(49∶51),detection wavelength was at 280 nm.Results The standard curves of baicalin was the linear over the rang of 0.01～0.393 mg·mL~(-1) and the recovery was 96.02%(RSD=2.35%).Conclusion The method is accurate,sensitive,reproductive and can be used for quality control of Changyijie granules.

Key concepts: Baicalin, Chromatography, High-performance liquid chromatography, Chemistry, Methanol, Quality standard, Content determination, Organic chemistry

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