Simultaneous Determination of Four Flavonoids in Folium Apocyni Veneti by HPCE-DAD

OBJECTIVE To establish a high performance capillary electrophoresis method with diode array detection (HPCE-DAD) for simultaneous determination of rutin,isoquercitrin,hyperin and quercetin in folium apocyni veneti from different habitats,harvesting time and species.METHODS Based on the mode of capillary zone electrophoresis,45 mmol·L-1 borax was used as buffer solution (pH 9.2),uncoated fused silica capillary (64.5 cm×75 μm id) was used,separation voltage was 25 kV,detection wavelength was at 206 nm,column temperature was maintained at 25 ℃,and sample was injected at 5 000 Pa for 5 s.RESULTS Four flavonoids showed good linearity (r0.998 6) in the range of the tested concentration.The average recoveries of the method were in the rang of 95.34%-102.65%.CONCLUSION The method is simple,accurate and reproducible,and can be used for quality control of Folium Apocyni Veneti.