RP-HPLC determination of three flavones in Apocyni Veneti Folium and its Chinese traditional patent medicine

Objective:To establish an HPLC method for the determination of three flavones(baimaside,rutin,isoquercitrin) in Apocyni Veneti Folium and its Chinese traditional patent medicine.Methods:Apocyni Veneti Folium and its Chinese traditional patent medicine were extracted with methanol by ultrasound extraction;The chromatographic procedure was carried out with Diamosil C18(250 mm×4.6 mm,5 μm) column,gradient elution with the mobile phase of acetonitrile-0.1% acetic acid solution at a flow of 1.0 mL·min-1,the detection wavelength was 360 nm,and the column temperature was room temperature.Results:The linear ranges of baimaside,rutin and isoquercitrin were 0.4-10 μg(r=0.9996),0.01-0.25 μg(r=0.9998) and 0.075-1.875 μg(r=0.9997),respectively;The average recoveries(n=5) were 99.7%(RSD=0.9%),100.8%(RSD=3.2%)and 99.7%(RSD=2.2%),respectively.Conclusion:The method is simple,rapid,accurate and reproducible,which can offer credible quality control method for Apocyni Veneti Folium and its Chinese traditional patent medicine.