Rapid Propagation of Medical Plant Taiwan Anoectochilus formosanus

[Objective] The study aimed to supply seed sources for deeply researching Taiwan Anoectochilus formosanus.[Method] With stem segments having axillary buds of Taiwan A.formosanus as explants,the different media were used to induce clustered buds and conduct rooting induction so as to establish a set of rapid propagation system.[Result] The media added 1.0～4.0 mg/L 6-BA solely increased the proliferation multiple of A.formosanus clustered buds from 2.3 to 4.5 times.When 6-BA and 2,4-D were added at the same,the proliferation multiple of clustered buds increased obviously.When the concentrations of 6-BA and 2,4-D were 2.0 and 0.4 mg/L resp.,the proliferation multiple of clustered buds reached 8.1.1/10 MS medium was more suitable for rooting induction.The active carbon concentration smaller than 0.1% was favorable for the rooting and growth of tissue cultured seedlings,the time needed for taking root was shorter and the average rooting number had some increment,the plant grew healthily and the transplant survival rate was over 95% after seedling-hardening for 10～20 d.[Conclusion] The optimum medium was MS+1.0 mg/L KT +0.2 mg/L NAA +2.0 mg/L 6-BA+0.4 mg/L 2,4-D for inducing clustered buds of Taiwan A.formosanus and was 1/10 MS+0.5 mg/L NAA+5% banana juice+0.05% active carbon for their rooting inductions.