Development of high oil content lines of Brassica napus through isolated microspore culture

The effects of genotype,cultural density and inflorescence stage on embryo production from microspores of three high oil content lines were investigated.The results showed that there were significant genotypic differences for embryogenesis among these genotypes.But the appropriate density of microspore culture of these three materials was 3 buds/plate.The highest embryogenesis frequency was obtained from buds sampled one week after initial efflorescence.Analysis of oil content by the NIRS(near-infrared reflectance spectroscopy) showed that microspore culture was an effective approach for selecting high oil content DH lines.Among 40 DH lines derived from 1481 and 1489,oil content of two DH lines,WD33 and WD-34,reach above 47%.