A Study on the Technique for Microspore Culture of Rapeseed (Brassica napus L.) and Its Simplification

With 64 genotypes of Brassica napus L. breeding lines as materials,experiments were carried out to evaluate the effect of different genotypes,cold pretreatment on buds and simplification of microspore culture methods on the yield of embryo in microspore culture. The main findings are as follows:( 1) Genotype is the most important factor in microspore culture,for the 64 donor materials used,the yield of embryo ranged from 0 to 31. 23 / bud;( 2) Cold pretreatment on buds is beneficial to microspore embryogenesis,and with the extension of the pretreatment time,the yield of embryo increased first and then decreased.( 3) Simplification of separation lotion formula has no significant effect on the yield of embryo.( 4) The percentage of microspore enlargement after one day's heat shock culture is a reference index for embryogenesis.