2009•Chih Wu Sheng Li Hsueh T'ung HsunRequires access

Obtainment and Characterization of DH Lines of A Rapid-cycling Brassica napus L.

WU Mian

Abstract

Tweety-three regenerated plants were recovered by microspore culture of a rapid-cycling Brassica napus.Among them,ten plants were diploid and thirteen were haploid after ploidy characterization.The DH(doubled haploid) lines were gained by treatment with colchicine.We generated completely homozygous DH lines as a model system for functional genomics work in B.napus.

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Key concepts: Brassica, Microspore, Ploidy, Doubled haploidy, Rapid cycling, Biology, Colchicine, Botany