ScholarLens Back to search
2005•Journal of Zhejiang University of TechnologyRequires access

RP-HPLC determination of the berberine type alkaloids from the Rhizoma coptidis

Yaqin Li

Open publisher page 0 citations

Abstract

A reversed-phase HPLC method was developed for the quantitative determination of Berberine type alkaloids form Rhizoma coptidis by 20% acetic acid. An ODS column was used, with the mobile phase consisted of methyl alcohol and borax-boric acid mixture ( 0.02 mol/L borax, adjust pH to 8.5 with boric acid ) (80:20),containing 0.2 mL two ethanol amine .the detecting wave-length is a 254 nm .Four kinds of berberine type alkaloids, berberine, berbamine, palmatine, jatrorrhizine can be separated completely. The regression equation is Y=25214.14X R=0.9993,and the average recovery is 99.485%. The method can be used for the research of Rhizoma coptidis and for the routine analysis of the crude drugs and Chinese traditional medicine containing berberine.

About this research paper

What this paper is about

A reversed-phase HPLC method was developed for the quantitative determination of Berberine type alkaloids form Rhizoma coptidis by 20% acetic acid. An ODS column was used, with the mobile phase consisted of methyl alcohol and borax-boric acid mixture ( 0.02 mol/L borax, adjust pH to 8.5 with boric acid ) (80:20),containing 0.2 mL two ethanol amine .the detecting wave-length is a 254 nm .Four kinds of berberine type alkaloids, berberine, berbamine, palmatine, jatrorrhizine can be separated completely. The regression equation is Y=25214.14X R=0.9993,and the average recovery is 99.485%. The method can be used for the research of Rhizoma coptidis and for the routine analysis of the crude drugs and Chinese traditional medicine containing berberine.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

A reversed-phase HPLC method was developed for the quantitative determination of Berberine type alkaloids form Rhizoma coptidis by 20% acetic acid. An ODS column was used, with the mobile phase consisted of methyl alcohol and borax-boric acid mixture ( 0.02 mol/L borax, adjust pH to 8.5 with boric acid ) (80:20),containing 0.2 mL two ethanol amine .the detecting wave-length is a 254 nm .Four kinds of berberine type alkaloids, berberine, berbamine, palmatine, jatrorrhizine can be separated completely. The regression equation is Y=25214.14X R=0.9993,and the average recovery is 99.485%. The method can be used for the research of Rhizoma coptidis and for the routine analysis of the crude drugs and Chinese traditional medicine containing berberine.

Key concepts: Berberine, Jatrorrhizine, Palmatine, Chemistry, Chromatography, Alkaloid, Borax, High-performance liquid chromatography

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
RP-HPLC determination of the berberine type alkaloids from the Rhizoma coptidis — Research Paper | ScholarLens