Simultaneous determination of berberine-type alkaloids in three kinds of coptidis rhizoma using RP-HPLC

Objective To establish a quantitative method for analyzing the concentration of berberine-type alkaloids in three kinds of coptidis rhizoma in a simultaneous manner using RP-HPLC,and find the different quantity of jatrorrhizine,palmatine,berberine in coptidis rhizoma from Yunnan,Sichuan and north America.Methods Angilent C 18,a mixed mobile phase of acetonitrile-water,and UV detection wavelength:345nm was employed to simultaneously determine the concentration of berberine-type alkaloids in three kinds of coptidis rhizoma.Results The average concentration of three berberine-type alkaloids in coptidis rhizoma was measured at 5.84%,1.26%,0.88%; 6.15%,1.35%,0.95%; 5.78%,1.38%,1.06%. The average recovery rates were registered respectively at 103.8%,103.8%,117.4%.Conclusion This method is simple,accurate,reliable,quick and reproducible. It can be used for quantitative analysis of three berberine-type alkaloids in coptidis rhizoma in a simultaneous manner.