SEROLOGICAL TEST OF ERYTHEMATOSUS PATIENTS' ANA,ANTI DS-DNA AND ANTIENA POLYPEPTIDE ANTIBODIES

[Objective] To explore the clinical significance of serological detection of ANA,anti ds-DNA and anti-ENA polypeptide antibodies,and the diagnosis value for SLE.[Methods] 80 patients with SLE in our hospital from January 2008December 2009 were selected as the observation group,60 healthy cases as control group during the same period.Indirect immunofluorescence,colloidal gold dot penetration method and Western blot were used to detect the positive rates of ANA,anti ds-DNA and anti-ENA in two groups.[Results] The positive rate of ANA,anti ds-DNA and anti-ENA antibodies was significantly higher in the observation group than which in the control group,the difference was significant(P﹤0.01);Sm,antiSS-A,anti SS-B,anti-nRNP,anti-rRNP of anti-ENA antibody spectrum were significantly higher than which in the control group,the difference was significant(P﹤0.01 or P﹤0.05).[Conclusion] ANA,anti ds-DNA and anti-ENA in the diagnosis of SLE are complementary.Joint testing will help improve the detection rate and accuracy of the diagnosis of SLE.Efficacy evaluation and prognosis have important clinical value,worthy of further clinical spread the applications.