Expression of Transgelin gene in myoma of uterus and its clinical significance

OBJECTIVE: To investigate the expression of Transgelin in uterine myoma tissues and to reveal its role in genesis and progression of uterine myoma,soas to provide a theoretical basis for new conservative means to treat uterine myoma.METHODS: Reverse transcription polymerase chain reaction(RT-PCR) and Western Blot were used to assess the expression of Transgelin mRNA and protein in 30 cases of uterine myoma tissues,30 cases of normal myometrium tissues which were over 2 cm far away the wrapping mambrane of myoma(as control) and 10 cases of normal myometrium tissues without myoma.RESULTS:The positive expression and relative content of Transgelin mRNA in uterin myoma tissues(23.3%,50.57±20.16) were statistically lower than those in the control(100.0%,84.62±6.12) and the normal myometrium tissues without myoma(100.0%,85.99±6.99),P0.001.No significant differences between those in the control and in the normal myometrium tissues without myoma(P0.05).The positive expression and relative content of Transgelin protein in myometrium tissues(16.7%,23.46±16.54) were statistically lower than those in the control(100.0%,61.08±8.73) and the normal myometrium tissues without myoma(100.0%,67.19±7.62),P0.001.No significant differences between those in the control and in the normal myometrium tissues without myoma(P0.05).CONCLUSION: Transgelin may play a suppression role in genesis of uterine myoma,and be relative to the genesis,progression and prognosis.