Study of Lead Stress on Mitotic Chromosome Aberration of Broad Bean Root Tip

Using broad bean root tip micro nuclear technology,micronucleus changes,chromosome aberration types of broad bean under the stress of lead were studied.Results showed that under the stress of heavy metal,cell micronucleus rate and concentration had proportion,and with the increase of concentration,the proportion of multi-core and cell micronucleus at different split phase immediately also increased.Cell division index and the concentration had inverse proportion,and types of chromosome aberration was also relatively rich with increase of the concentration.Chromosome fragment,lagging chromosomes,chromosome bridge,multipolar distribution of chromosome conglutination,the types of abnormal chromosome unwinding and chromosome doubling under the stress of lead was clearly observed.The result further confirmed that the lead had poisoning effect on plant cells.