Influence of Coccinellin Concentration on Caryomitotic in Root Tip Cells of Broad Bean

[Objective]The purpose was to supply basis for reasonable application of food additive coccinellin.[Method]After seed soaking and accelerating germination,the broad bean seeds were treated with food additive coccinellin at different concn.(concentration) and analytical pure coccinellin for 24 h,with distilled water treatment as CK,the samples were fixed,dyed and squashed for microscopic examination.The micronucleus permillage and chromosomal aberration percentage in root tip cells were calculated.[Result]When coccinellin concen.were 0.02 and 0.04 g/L resp.,the color of newly grown root of broad bean was bright and had no difference from that of CK.As the coccinellin concen.was increased,the color of broad bean root showed variation trend of yellow-brown-black.Both the 2 kinds of coccinellin could induce the root tip cells to generate micronucleus and chromosomal aberration and their micronucleus permillage and chromosomal aberration percentage were increased along with the ascending of coccinellin concen.Most of the micronucleus permillage and chromosomal aberration percentage in cells induced by analytical pure coccinellin were lower than that induced by food additive coccinellin.[Conclusion]The application of excessive coccinellin would generate toxicity against organisms.