Effects of Irradiation with Low-energy Nitrogen Ion Injection on Root Tip Cells of Broad Bean

In order to study the cytogenetic effects of low-energy nitrogen ion irradiation,broad bean seed embryo was irradiated by different doses of nitrogen ions.Micronucleus rate,mitotic index and chromosome aberration in root-tip cells were analyzed.The results showed that the injection of ions inhibited mitosis of root tip cells,interfered the normal process of mitosis,caused aberrations of chromosome structure,behavior and number.The frequency of micronucleus and chromosomal aberrations increased with the increasing radiation dosage,while mitotic index decreased.