Experimental study of protective effects of Chinese baicalin on human periondontal ligament fibroblasts

Objective To observe the effects of the Chinese baicalin on LPS which lead to the decreases of activities and ultrastructural impairments of human periodontal ligament fibroblasts (HPLFs), and try to study the mechanism of the effects.Methods The cell culture technique in vitro, thiazolyl blue tetrazolium bromide (MTT) staining and spectrophotometric assay and transmission electron microscope (TEM) method were used to observe the effects of baicalin on LPS.Results At the concentration of 100μg/mL, LPS of E. Coli could inhibit the proliferation of activities of HPLFs remarkably and proliferation of activities of HPLFs improved greatly after adding baicalin. Furthermore, LPS had significant impairmental effect on ultrastrutures of HPLFs in 100μg/ml, especially on the mitochondria. After adding baicalin, the impairment of ultrastructures of HPLFs was decreased.Conclusion The Chinese baicalin has the protective effects on the fibroblasts of human periodontal ligament induced by LPS. The mechanism of the effects may be related to the decomposition of LPS and the protection of the Chinese baicalin which prevents LPS from going into HPLFs.