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2010•Chongqing yixueRequires access

Effects of Baicalin on apoptosis of cultured HSC

Zhou Xian

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Abstract

Objective To study the effects of Baicalin on the apoptosis of cultured rat hepatic stellate cells(HSCs).Methods HSCs were cultured with different concentrations of Baicalin 0,75,150,300,600,1 200 μg/mL for 24 hours.Cell apoptosis was detected with phase contrast light microscopy,MTT colorimetric assay,AO/EB fluorescent staining and flow cytometry.Results Baicalin markedly increases apoptosis of HSCs,and the effect depends on the drug dose.Conclusion Baicalin can cause HSCs apoptosis directly,which might be one of the mechanisms of Baicalin in reducing liver fibrosis.

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What this paper is about

Objective To study the effects of Baicalin on the apoptosis of cultured rat hepatic stellate cells(HSCs).Methods HSCs were cultured with different concentrations of Baicalin 0,75,150,300,600,1 200 μg/mL for 24 hours.Cell apoptosis was detected with phase contrast light microscopy,MTT colorimetric assay,AO/EB fluorescent staining and flow cytometry.Results Baicalin markedly increases apoptosis of HSCs,and the effect depends on the drug dose.Conclusion Baicalin can cause HSCs apoptosis directly,which might be one of the mechanisms of Baicalin in reducing liver fibrosis.

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Available abstract

Objective To study the effects of Baicalin on the apoptosis of cultured rat hepatic stellate cells(HSCs).Methods HSCs were cultured with different concentrations of Baicalin 0,75,150,300,600,1 200 μg/mL for 24 hours.Cell apoptosis was detected with phase contrast light microscopy,MTT colorimetric assay,AO/EB fluorescent staining and flow cytometry.Results Baicalin markedly increases apoptosis of HSCs,and the effect depends on the drug dose.Conclusion Baicalin can cause HSCs apoptosis directly,which might be one of the mechanisms of Baicalin in reducing liver fibrosis.

Key concepts: Baicalin, Apoptosis, Hepatic stellate cell, Flow cytometry, Chemistry, MTT assay, Molecular biology, Staining

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