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Tissue culture and plant regeneration of Petunia hybrida

Hong Quan, Ying Qi, Heping Shi

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Abstract

The light green compact callus was developed from leaf explant of Petunia hybrida var. grandiflora when cultured on MS medium supplemented with 6-BA 1.0mg/L + NAA 0.1 mg/L for 3 weeks. When calli were transferred into the differentiation medium, MS + 6-BA 0.5 mg/L + 4-PU 0.5 mg/L + NAA 0.1mg/L for 1 week, adventitious buds were formed. Transferred the buds with 3cm high into the root induction medium (1/2MS+NAA0.1mg/L+GA3 0.5mg/L) for 10d, roots were induced.

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What this paper is about

The light green compact callus was developed from leaf explant of Petunia hybrida var. grandiflora when cultured on MS medium supplemented with 6-BA 1.0mg/L + NAA 0.1 mg/L for 3 weeks. When calli were transferred into the differentiation medium, MS + 6-BA 0.5 mg/L + 4-PU 0.5 mg/L + NAA 0.1mg/L for 1 week, adventitious buds were formed. Transferred the buds with 3cm high into the root induction medium (1/2MS+NAA0.1mg/L+GA3 0.5mg/L) for 10d, roots were induced.

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Available abstract

The light green compact callus was developed from leaf explant of Petunia hybrida var. grandiflora when cultured on MS medium supplemented with 6-BA 1.0mg/L + NAA 0.1 mg/L for 3 weeks. When calli were transferred into the differentiation medium, MS + 6-BA 0.5 mg/L + 4-PU 0.5 mg/L + NAA 0.1mg/L for 1 week, adventitious buds were formed. Transferred the buds with 3cm high into the root induction medium (1/2MS+NAA0.1mg/L+GA3 0.5mg/L) for 10d, roots were induced.

Key concepts: Explant culture, Petunia, Callus, Tissue culture, Botany, Biology, Regeneration (biology), Murashige and Skoog medium

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