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TISSUE CULTURE OF COLORED COMMON CALLALILY (PETUNIA HYBRIDA)

Qu SuPing

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Abstract

Shoot tips, stem segments and young leaves of Petunia hybrida were adopted as explants and cultured on MS medium supplemented with different combinations of plant growth regulators. Best results of callus induction were achieved on MS enriched with BA 2 mg/L and NAA 0.1 mg/L. The calli were then transferred onto MS supplemented with BA 1 mg/L and NAA 0.1 mg/L for shoot differentiation. Repeated operation of the two procedures gave the highest propagation coefficient, induction rate and differentiation percentage, and the least aberration. Optimum media were MS + NAA 0.5 mg/L and MS + NAA 0.3 mg/L + IAA 0.2 mg/L.

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What this paper is about

Shoot tips, stem segments and young leaves of Petunia hybrida were adopted as explants and cultured on MS medium supplemented with different combinations of plant growth regulators. Best results of callus induction were achieved on MS enriched with BA 2 mg/L and NAA 0.1 mg/L. The calli were then transferred onto MS supplemented with BA 1 mg/L and NAA 0.1 mg/L for shoot differentiation. Repeated operation of the two procedures gave the highest propagation coefficient, induction rate and differentiation percentage, and the least aberration. Optimum media were MS + NAA 0.5 mg/L and MS + NAA 0.3 mg/L + IAA 0.2 mg/L.

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Available abstract

Shoot tips, stem segments and young leaves of Petunia hybrida were adopted as explants and cultured on MS medium supplemented with different combinations of plant growth regulators. Best results of callus induction were achieved on MS enriched with BA 2 mg/L and NAA 0.1 mg/L. The calli were then transferred onto MS supplemented with BA 1 mg/L and NAA 0.1 mg/L for shoot differentiation. Repeated operation of the two procedures gave the highest propagation coefficient, induction rate and differentiation percentage, and the least aberration. Optimum media were MS + NAA 0.5 mg/L and MS + NAA 0.3 mg/L + IAA 0.2 mg/L.

Key concepts: Explant culture, Petunia, Shoot, Callus, Tissue culture, Botany, Murashige and Skoog medium, Biology

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