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Tissue Culture and Rapid Propagation of Petunia hybrida

Zhang Rui-yue

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Abstract

With the leaf of Petunia hybrida as explant,the effect of medium adding different hormone component with different concentration on cespitose bud and rooting was studied.Results showed that the optimum bud-inducing medium was MS+6-BA 2.0 mg/L + NAA 0.3 mg/L and the most suitable rooting medium was 1/2MS+NAA 0.05～0.1 mg/L.The intact regeneration seedlings were formed after the explants cultured for 40 d.

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What this paper is about

With the leaf of Petunia hybrida as explant,the effect of medium adding different hormone component with different concentration on cespitose bud and rooting was studied.Results showed that the optimum bud-inducing medium was MS+6-BA 2.0 mg/L + NAA 0.3 mg/L and the most suitable rooting medium was 1/2MS+NAA 0.05～0.1 mg/L.The intact regeneration seedlings were formed after the explants cultured for 40 d.

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Available abstract

With the leaf of Petunia hybrida as explant,the effect of medium adding different hormone component with different concentration on cespitose bud and rooting was studied.Results showed that the optimum bud-inducing medium was MS+6-BA 2.0 mg/L + NAA 0.3 mg/L and the most suitable rooting medium was 1/2MS+NAA 0.05～0.1 mg/L.The intact regeneration seedlings were formed after the explants cultured for 40 d.

Key concepts: Explant culture, Petunia, Tissue culture, Biology, Botany, Murashige and Skoog medium, Horticulture, Regeneration (biology)

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