Study on Induced Differentiation of Petunia hybrida Shoot Tip

[Objective] The rapid and economic production of Petunia hybrida sapling would be realized through the research on the industrialization of its tissue culture.[Method] The introduction and regeneration of the Petunia hybrida stem-tip culturing in 5 media were experimented and the effect of the concentration of NAA on the survival rate of Petunia hybrida plantlet was researched.[Results] The concentration of NAA greatly affected the survival rate of the explants and the best concentration of NAA in introduction medium was 0.5 mg/L,then 0.7 mg/L or 0.3 mg/L,and the worst,0.1 mg/L or 0.9 mg/L.The best suitable regeneration medium was MS+NAA 0.5 mg/L+6-BA 0.3 mg/L+agar 8 g/L+sucrose 30 g/L.[Conclusion] The result could be referred for the Petunia hybrida tissue culture.