Kinetic Model for Enzymatic Hydrolysis of Decapterus Maruadsi Protein

In order to find out the process condition for preparing the bioactive peptides obtained from Decapterus maruadsi protein,the dynamic model for the Decapterus maruadsi enzymatic system was studied.Alkaline protease was used to hydrolyze Decapterus maruadsi protein which forms complicated active peptides consequently.The dynamic model for enzymolysis of Decapterus maruadsi protein alkaline protease system was obtained via the pH-stat method.The molecular weight distribution of the polypeptide fragments under the different degrees of hydrolysis(DH) in the hydrolysate of Decapterus maruadsi protein was analyzed by high performance size exclusion chromatography(HPSEC).In terms of chromatograms obtained at different DH values,a 3-D continuous surface and corresponding contour were plotted to characterize the relation of DH polypeptide fragment molecular weight-mass percent in the enzymatic process.Mass percentages of polypeptide fragments at the different molecular weights and DH values were established with Matlab simulation.Results show that the 3-D dynamic model is basically consistent with the real hydrolysis.This model has revealed the composition variation with polypeptide molecular weights in the enzymolysis and may be used to conduct the real-time quantitative analysis and the dynamic attribute of the enzymolysis system.It is also beneficial to the directional and quantitative preparation of target active peptides.