Study on Technology of Preparing Oligopeptide from Decapterus maruadsi Protein by Enzymatic Hydrolysis

This study focused on the preparing oligopeptide from Decapterus Maruadsi protein by enzymatic hydrolysis.Response Surface Methodology(RSM) was used to gain the optimal parameter of processing and to discuss how the parameters influenced the extraction rate of polypeptide.As a results indicated that the optimal parameter of processing were as follows: the effect of hydrolysis was the best by adding papain protease(the enzyme concentration was 0.10%),liquid-to-solid ratio 2:1(m:m),hydrolysis temperature 54.4(℃),hydrolysis time 3.18(h),the extraction rate of polypeptide 32.35% by experiment and the recovery rate of protein was 98.24%.