2005•Yiyao daobaoRequires access

Determination of Paeoniflorin in Rupineixiao Oral Liquid with Reverse-phase High Performance Liquid Chromatography(RP-HPLC)

Wenqing Wang

Abstract

Objective To set up a reverse-phase high performance liquid chromatography( RP-HPLC) method for the determination of the content of paeoniflorin in rupineixiao oral liquid. Methods The chromatographic column was a YMC-Pack ODS (4.6 mm×250 mm,5μm). The mobile phase was consisted of methanol-water- glacial acetic acid ( 25∶75∶(0.2)) ,and the detecting wave-length was 230 nm. The column was kept at room temperature. Results The linear relationship was fairly good when the amount of paeoniflorin added was within the range of 0.201-3.348 μg (r=0.999 9) . The average rate of recovery was 98.60% ,RSD=1.70%(n=6). Conclusion The method was shown to be suitable for the determination of the content of paeoniflorin in rupineixiao oral liquid

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Key concepts: Paeoniflorin, Chromatography, High-performance liquid chromatography, Chemistry, Acetic acid, Methanol, Phase (matter), Biochemistry