Correlation of Detection of HBV-DNA by Fluorescent Quantitative PCR with Routine Detection of HBV

Objective:To explore the relationship between detection of HBV-DNA and routine detection of HBV.Method:HBV-DNA was detected with fluorescent quantitative PCR(FQ-PCR) and HBV was routinely determined with ELISA in serum samples from 307 suspected cases of hepatitis B.Meanwhile,the correlation of results between the two methods was analyzed.Result:The detecting rate of HBV-DNA was 98.15%(53/54) and the mean copy number was 7.56E+06/ml in patients of HBsAg(+),HBeAg(+) and HBcAb(+)(group A),27.78%(15/54)and 3.79E+05/ml in patients of HBsAg(+),HBeAb(+) and HBcAb(+)(group B),91.18%(31/34)and 2.85E+05/ml in patients of HBsAg(+) and HBcAb(+)(group C).The 2 parameters were significantly lower in group B than in group A(P0.01 and 0.05).Furthermore,they were respectively 1.6%(1/63) and 1.13E+08/ml in patients of HBsAb(+),16.67%(1/6) and 3.00E+04/ml in patients of HBcAb(+),12.50%(4/32) and 3.00E+05/ml in patients of HBsAb(+),HBeAb(+) and HBcAb(+),0(0/2) in patients of HBsAg(+) and 1.61%(1/62)and 2.75E+05/ml in HBV-M-negative patients.Conclusion:HBV-DNA positivity may appear in HBV-M-negative patients.Therefore,the 2 methods should be simultaneously used in the detection of HBV infection to promote the detecting rate to provide more reliable evidence for judgment of HBV infection,duplication and infectivity and its treatment in clinical practice.