Determination of ginsenoside Rg1 and Re in Youganning capsule by HPLC
Liyan Zhou
Abstract
Liyan Zhou
Abstract
Objective To establish the quantitative method of ginsenoside Rg1 and Re in Youganning capsule by RP-HPLC.Method Samples were analyzed on ODS C_(18) column.The mobile phase consisted of acetonitrile-0.05%H_3PO_4(19.7∶80.3) eluted in gradient mode.UV detector was set at wavelength of 203 nm.Result The assay was linear over the range of 1.06-10.60 μg for ginsenoside Rg1 and 0.848-8.48 μg for ginsenoside Re.The average recovery was 100.09% with RSD of 2.15%(n=6).Conclusion This method is rapid,sensitive and reproducible for the quality control of Youganning capsule.
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Objective To establish the quantitative method of ginsenoside Rg1 and Re in Youganning capsule by RP-HPLC.Method Samples were analyzed on ODS C_(18) column.The mobile phase consisted of acetonitrile-0.05%H_3PO_4(19.7∶80.3) eluted in gradient mode.UV detector was set at wavelength of 203 nm.Result The assay was linear over the range of 1.06-10.60 μg for ginsenoside Rg1 and 0.848-8.48 μg for ginsenoside Re.The average recovery was 100.09% with RSD of 2.15%(n=6).Conclusion This method is rapid,sensitive and reproducible for the quality control of Youganning capsule.
Key concepts: Ginsenoside Rg1, Capsule, Chromatography, Gradient elution, Chemistry, Ginsenoside, High-performance liquid chromatography, Linear range