2005•Unpublished venueRequires access

Quantitative Determination of the Content of Ginsenoside Re in Guchang Capsules by Reversed-phase HPLC

Chi Hai-dong

Abstract

Objective To develop a quantitative determination method of the content of ginsenoside Re in Guchang Capsules.Methods A reversed-phase high performance liquid chromatographic method was employed and the measurement conditions used are as follows:Apollo C18(250 mm×4.6 mm,5 μm)column;mobile phase:acetonitrile-0.05% phosphoric acid(21∶79);the detection wavelength was set at 203 nm.Results The linear range of ginsenoside Re is 0.508～12.192 μg.The average recovery Re was 101%(RSD=1.5%).Conclusions The method is helpful to the quality control of ginsenoside Re in Guchang Capsules.

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Key concepts: Chromatography, Phosphoric acid, High-performance liquid chromatography, Ginsenoside, Content determination, Chemistry, Ginsenoside Rg1, Quantitative analysis (chemistry)