Quantitative Determination of Chlorogenic Acid and Baicalin in Shuanghuanglian Powder Injection by HPLC

Objective To develop an RP-HPLC method for the determination of chlorogenic acid and baicalin in Shuanghuanglian powder injection.Methods A C_8～C_(13)mixedalkyl(5μm,4.6 mm×150 mm)bond silica for reversed-phase HPLC was prepared.The mobile phase was CH_3OH―phosphoric acid solution(pH 2.7).The flow rate was set at 1.0 ml/min.The detection wavelength was at 323 nm.Chlorogenic acid and baicalin were separated by HPLC with grade elution.Results The linearity ranges of chlorogenic acid and baicalin were 4.0～100.0 mg /ml and 8.6～215.0 mg /ml respectively,the correlation coefficients were 0.999 89 and 0.999 97,the average recoveries of adding sample were 100.53% and 99.94%,detection limits were 0.014 mg/ml and(0.020) mg /ml,the RSD(n=5) of measurement precision test were 1.12% and 0.61%,the RSD(n=5) of reproducibility between tests were 0.89% and 0.54%.The content of chlorogenic acid in Shuanghuanglian powder was 1.577～1.753 mg/ml,and that of baicalin was 26.02～28.68 mg/ml.Conclusion The method is simple,fast,accurate,sensitive,reproducible and low cost.It is fit for the quality control of Shuanghuanglian powder.