Quantitative Determinnation of Chlorogenic Acid and Baicalin incompound Qinlan oral liquid by HPLC

Objective To develop an RP-HPLC method for the determination of chlorogenic acid and baicalin in compound Qinlan oral liquid.Methods A C8-C13 mixedalkyl(150 mm×4.6 mm,5 μm)bond silica for reversed-phase HPLC was prepared.The mobile phase was CH3OH-0.4% Phosphoric acid.The flow rate was set at 1.0 mL/min.The detection wavelength was at 323 nm.Chlorogenic acid and baicalin were separated by HPLC with grade elution.Results The linearity ranges of chlorogenic acid and baicalin were 4.1-102.0 μg/mL and 8.5-212.6 μg/mL respectively,the correlations coefficients were 0.999 2 and 0.999 3,the average recoveries of adding sample were 97.58 % and 98.16 %,the detection limits were 0.02 μg/mL and 0.05 μg/mL,the RSD of measurement precisions test were 1.10% and 1.88%,the RSD of reproducibility between tests were 1.35% and 1.00%,the RSD of stability test were 1.34% and 1.18%.The content of chlorogenic acid in compound Qinlan oral liquid was 0.331-0.344 mg/mL,and that of baicalin was 8.778-10.44 mg/mL.Conclusion The method is simple,fast,accurate,sensitive,reproducible and low cost.It is fit for the quality control of compound Qinlan oral liquid.