Quantitative Determinnation of Chlorogenic Acid and Baicalin inQingkailing Granule by HPLC

Objective To develop an RP-HPLC method for the determination of chlorogenic acid and baicalin in Qingkailing Granule.Methods A C18(150mm×4.6mm,5μm) bond silica for reersed-phase HPLC was prepared.The mobile phase was CH3OH-0.4% Phosphoric acid.The flow rate was set at 1.0mL/min.The detection wavelength was at 323 nm.Chlorogenic acid and baicalin were separated by HPLC with grade elution.Results The linearity ranges of chlorogenic acid and baicalin were 4.248-106.2μg/mL and 9.224-203.6μg/mL respectively;The correlation coefficient were 0.9997 and 0.9998;The recoveries of adding sample rate were 101.23% and 100.72%;The detection limits were 0.048μg and 0.034μg.The RSD(n=5) of measurement precisions test were 0.75% and 1.88%.The RSD(n=5) of reproducibility between tests were1.99% and 4.73%.The RSD(n=5) of stability test were 1.8% and 0.52% The content of chlorogenic acid in compound Qingkailing granule was 0.2215%-0.2594%,and that of baicalin was 4.0947%-4.3743%.Conclusion The method is simple,fast,accurate,sensitive,reproducible and low cost.It is fit for the quality control of compound Qingkailing Granule.