Development of One Step RT-PCR Assay to Detect CSFV

Based on E2 gene sequences of CSFV Shimen published on NCBI,a pair of primers were designed and synthesized for detecting E2 gene.E2 gene was amplified by one step RT-PCR from total RNA,which had been extracted from tissues of pigs doubting infected by classical swine fever virus and PK-15 infected by CSFV F114.We compared IF method with one step RT-PCR which had been established.24 samples of 26 samples doubting infected by CSFV were positive deteced by one step RT-PCR,the positive rate of one step RT-PCR was 92.3%.Results showed that the RT-PCR is specific and sensitive,and can be used as a method to identify CSFV isolates,detect samples of clinical cases and investigate molecular epidemiology of CSFV.