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2006•Heilongjiang Bayi Nongken Daxue xuebaoRequires access

Rapid Detection of Classical Swine Fever Virus via Single-step RT-PCR

Wang Chun-ming

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Abstract

A single-step RT-PCR assay for detection of classical swine fever virus (CSFV) was developed using a pair of primers designed from the conserved sequence of gene-unencoding region at 5’ terminal of the virus genome. The results indicated that this single-step RT-PCR assay was highly specific, sensitive and easy for earlier diagnosis of classical swine fever and identification of CSFV.

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What this paper is about

A single-step RT-PCR assay for detection of classical swine fever virus (CSFV) was developed using a pair of primers designed from the conserved sequence of gene-unencoding region at 5’ terminal of the virus genome. The results indicated that this single-step RT-PCR assay was highly specific, sensitive and easy for earlier diagnosis of classical swine fever and identification of CSFV.

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Available abstract

A single-step RT-PCR assay for detection of classical swine fever virus (CSFV) was developed using a pair of primers designed from the conserved sequence of gene-unencoding region at 5’ terminal of the virus genome. The results indicated that this single-step RT-PCR assay was highly specific, sensitive and easy for earlier diagnosis of classical swine fever and identification of CSFV.

Key concepts: Classical swine fever, Virology, Virus, Biology, Real-time polymerase chain reaction, Polymerase chain reaction, Gene, Gene sequence

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