2003•Zhongguo shouyi ke-jiRequires access

Study on relationship between RT-PCR and rabbit-cross test for detection of classical swine fever virus

Jianzhu Liu

Abstract

Relationship between RT-PCR and rabbit-cross test for detection of classical swine fever virus (CSFV) was studied in this experiment. One set of specific primers were designed according to the conserved sequence of E2 gene of CSFV in GenBank and a standard RT-PCR was founded to detect the infection of CSFV. Compared with rabbit-cross test, 7 samples from pigs suspected infection with CSFV were detected with RT-PCR. It was indicated that the results from the detection of CSFV by RT-PCR coinside completely with that from the detection with rabbit-cross test, and that RT-PCR detection can save plenty of time.

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Key concepts: Classical swine fever, Biology, GenBank, Virology, Virus, Real-time polymerase chain reaction, Gene sequence, Pcr test