Effect of As_2O_3 on Gene Expression of VEGF and bFGF in ACC-M Cells

Objective:The aim of this study was to investigate the proliferation effects of As2O3 on salivary adenoid cystic carcinoma-M(ACC-M) cells in vitro and gene expression of VEGF and bFGF within those cells.The possible mechanism of As2O3 on gene expression of VEGF and bFGF was also elucidated.Methods:ACC-M cells were treated with different concentration of As2O3 for different time.Cytomorphology of ACC-M cells was observed on phasecontrast microscope.Proliferation or suppression effect on cells' viability was detected by MTT method.Gene and protein expression of VEGF and bFGF were investigated by RT-PCR and Western blot analysis respectively.Results:Cell viability after As2O3 treatment was markedly suppressed and exhibited as a dose-and time-dependent pattern.RT-PCR revealed no statistically difference between gene expression of VEGF and bFGF.Through Western blot analysis,a negative correlation between As2O3 concentration and amount of protein product of these two cytokines was determined.Conclusions:As2O3 might markedly suppressed ACC-M cells' viability with a time-dose reliable pattern in vitro.As2O3 decreases protein product of VEGF and bFGF apparently.Therefore,the agent might suppress vessel formation of ACC-M cells.