Effect of AS_2O_3 on Gene Expression of VEGF and bFGF in ACC-2 Cells Line

Objective:To investigate the effect of As_2O_3 on salivary adenoid cystic carcinoma-2(ACC-2)ceils proliferation in vitro and expression of VEGF and bFGF of the cells.Methods:ACC-2 cells were cultured with As_2O_3 in different concentrations(0、2、4、6μmol/L)for different time(24h、48h、72h).Cytomorphology was observed by phasecontrast microscopy.As_2O_3 cytotoxity was deter-mined by MTr assay.Gene and protein expression of VEGF and bFGF were investigated by RT-PCR and Western blotting analysis respectively.Results:Proliferation of ACC-2 cells were markedly promoted 24h after As_2O_3 administration.However,As_2O_3 induced a dose-and time-dependent tumor cell proliferation. The results of RT-PCR revealed no significant difference between gene expression of VEGF and bFGF.A negative relationship between As_2O_3 concentration and protein product of these two cytokines were observed.Conclusion:(1)ACC- 2 ceils might be markedly depressed after 48 h by As_2O_3 administration in a time-and dose-dependenty reliable pattern. In contrast,the cells' proliferation could be promoted within 48 h.(2)As_2O_3 toxicity can decrease protein product of VEGF and bFGF apparently.So might depress vessel formation of ACC-2 cells.