ScholarLens Back to search
2005•Zhongguo linchuang yaolixue yu zhiliaoxueRequires access

China cobra venom active factor (CCVAF) inhibits VEGF and bFGF in lung cancer cell line H1299

YU Hong-e

Open publisher page 0 citations

Abstract

AIM: To study the effect of China cobra venom active factor (CCVAF) on the expression of VEGF and bFGF in human non-small cell lung cancer cell line H1299. METHODS: VEGF and bFGF mRNA level was measured by RT-PCR. VEGF and bFGF protein level in the supernatant was examined by ELISA. RESULTS: The mRNA expression level of bFGF was also reduced after treatment with the CCVAF in dose-dependent manner. Giving the CCVAF 4.0 μg·ml~ -1 to cell H1299 for 4 hours could notablely decreased the mRNA expression of bFGF while it had no such effect on VEGF mRNA expression level. The content of VEGF, and bFGF in supernatant of H1299 cells were significantly decreased after treatment for 24 hour with CCVAF in dose-dependent manner. There was significant difference compared with control group (P 0.05). CONCLUSION: CCVAF inhibits the expression of VEGF and bFGF in H1299 cells.

About this research paper

What this paper is about

AIM: To study the effect of China cobra venom active factor (CCVAF) on the expression of VEGF and bFGF in human non-small cell lung cancer cell line H1299. METHODS: VEGF and bFGF mRNA level was measured by RT-PCR. VEGF and bFGF protein level in the supernatant was examined by ELISA. RESULTS: The mRNA expression level of bFGF was also reduced after treatment with the CCVAF in dose-dependent manner. Giving the CCVAF 4.0 μg·ml~ -1 to cell H1299 for 4 hours could notablely decreased the mRNA expression of bFGF while it had no such effect on VEGF mRNA expression level. The content of VEGF, and bFGF in supernatant of H1299 cells were significantly decreased after treatment for 24 hour with CCVAF in dose-dependent manner. There was significant difference compared with control group (P 0.05). CONCLUSION: CCVAF inhibits the expression of VEGF and bFGF in H1299 cells.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

AIM: To study the effect of China cobra venom active factor (CCVAF) on the expression of VEGF and bFGF in human non-small cell lung cancer cell line H1299. METHODS: VEGF and bFGF mRNA level was measured by RT-PCR. VEGF and bFGF protein level in the supernatant was examined by ELISA. RESULTS: The mRNA expression level of bFGF was also reduced after treatment with the CCVAF in dose-dependent manner. Giving the CCVAF 4.0 μg·ml~ -1 to cell H1299 for 4 hours could notablely decreased the mRNA expression of bFGF while it had no such effect on VEGF mRNA expression level. The content of VEGF, and bFGF in supernatant of H1299 cells were significantly decreased after treatment for 24 hour with CCVAF in dose-dependent manner. There was significant difference compared with control group (P 0.05). CONCLUSION: CCVAF inhibits the expression of VEGF and bFGF in H1299 cells.

Key concepts: Basic fibroblast growth factor, VEGF receptors, Messenger RNA, Vascular endothelial growth factor, Cell culture, Cell, Lung cancer, Venom

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
China cobra venom active factor (CCVAF) inhibits VEGF and bFGF in lung cancer cell line H1299 — Research Paper | ScholarLens