HPLC determination of Paeoniflorin in Qixueshuangbu Oral Liquid

Objective To establish a method for the determination of Paeoniflorin in Qixueshuangbu Oral Liquid,which could provide envidence of research for quality standard.Methods HPLC method was used with Hanbang ODS-C18 column(250 mm × 4.6 mm,5 μm) and a mobile phase of methanol-0.1% phosphoric acid solution(29∶71).Flow rate was 1.0 mL/min,column temperature was 25℃,the detection wavelength was setted at 230 nm and sample loop volume was 10 μL.Results The calibration curve was linear within the range of 10.00-80.00 μg/mL of Paeoniflorin,the average recovery was 99.45%(RSD=0.63%).Conclusion The method is simple,accurate and repeatable,which can be used for the quality control of Qixueshuangbu Oral Liquid.