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Determination of notoginsenoside R_1、ginsenoside Rg_1 and ginsenoside Rb_1 in Panax zingiberensis by HPLC

Wenyan Li

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Abstract

A method is established for the determination of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in Panax zingiberensis.A HPLC method was adopted.The analysis was carried out on all analytical column C18(50 mm×4.6 mm,luna 5 μm).The flow rate was 1.0 mL·min-1 and the detective wavelength was set at 203 mm with column temperature of 30 ℃.The mobile phase consisted of acetonitrile-water(gradient elution).The linear range was 0.315—1.575 μg(r=0.999 1)and the average recovery was 101.4%with the RSD of 1.79% for notoginsenoside R1.The linear range was 1.203—6.015 μg(r=0.999 8)and the average recovery was 98.54%with the RSD of 1.9% for ginsenoside Rg1.The linear range was 0.276—1.38 μg(r= 0.999 6)and the average recovery was 102.1%with the RSD of 1.53% for ginsenoside Rb1.The method is simple,reproducible and suitable for determination of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in Panax zingiberensis.

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A method is established for the determination of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in Panax zingiberensis.A HPLC method was adopted.The analysis was carried out on all analytical column C18(50 mm×4.6 mm,luna 5 μm).The flow rate was 1.0 mL·min-1 and the detective wavelength was set at 203 mm with column temperature of 30 ℃.The mobile phase consisted of acetonitrile-water(gradient elution).The linear range was 0.315—1.575 μg(r=0.999 1)and the average recovery was 101.4%with the RSD of 1.79% for notoginsenoside R1.The linear range was 1.203—6.015 μg(r=0.999 8)and the average recovery was 98.54%with the RSD of 1.9% for ginsenoside Rg1.The linear range was 0.276—1.38 μg(r= 0.999 6)and the average recovery was 102.1%with the RSD of 1.53% for ginsenoside Rb1.The method is simple,reproducible and suitable for determination of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in Panax zingiberensis.

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Available abstract

A method is established for the determination of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in Panax zingiberensis.A HPLC method was adopted.The analysis was carried out on all analytical column C18(50 mm×4.6 mm,luna 5 μm).The flow rate was 1.0 mL·min-1 and the detective wavelength was set at 203 mm with column temperature of 30 ℃.The mobile phase consisted of acetonitrile-water(gradient elution).The linear range was 0.315—1.575 μg(r=0.999 1)and the average recovery was 101.4%with the RSD of 1.79% for notoginsenoside R1.The linear range was 1.203—6.015 μg(r=0.999 8)and the average recovery was 98.54%with the RSD of 1.9% for ginsenoside Rg1.The linear range was 0.276—1.38 μg(r= 0.999 6)and the average recovery was 102.1%with the RSD of 1.53% for ginsenoside Rb1.The method is simple,reproducible and suitable for determination of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in Panax zingiberensis.

Key concepts: Ginsenoside Rg1, Ginsenoside, Chromatography, Chemistry, Gradient elution, High-performance liquid chromatography, Linear range, Content determination

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Determination of notoginsenoside R_1、ginsenoside Rg_1 and ginsenoside Rb_1 in Panax zingiberensis by HPLC — Research Paper | ScholarLens