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2003•Strait Pharmaceutical JournalRequires access

Determination of Puerarin in Niuge Oral Liquid by HPLC

Ye Dong

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Abstract

OBJECTIVE To establish a method for determination puerarin in Niuge oral Liquid by high perfomance Liquid chromatography.METHODS Nova pak C 18 column(3 9mm×150mm,4μm)as stationary phase,methanol H 2O=25∶75(V/V) as mobile phase the flow rate was 0 7mL·min -1 and the wavelength of detection was at 250nm.RESULTS The calibration curve was showed good linearity within the range of 0 404～2 020μg,r=0 9999,the average recovery was 99 23%,RSD=1 29%;and the RSD value of presity was 0 26%.CONCLUSION The method can eliminate interference of other components and is accurate,precise and simple.

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What this paper is about

OBJECTIVE To establish a method for determination puerarin in Niuge oral Liquid by high perfomance Liquid chromatography.METHODS Nova pak C 18 column(3 9mm×150mm,4μm)as stationary phase,methanol H 2O=25∶75(V/V) as mobile phase the flow rate was 0 7mL·min -1 and the wavelength of detection was at 250nm.RESULTS The calibration curve was showed good linearity within the range of 0 404～2 020μg,r=0 9999,the average recovery was 99 23%,RSD=1 29%;and the RSD value of presity was 0 26%.CONCLUSION The method can eliminate interference of other components and is accurate,precise and simple.

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Available abstract

OBJECTIVE To establish a method for determination puerarin in Niuge oral Liquid by high perfomance Liquid chromatography.METHODS Nova pak C 18 column(3 9mm×150mm,4μm)as stationary phase,methanol H 2O=25∶75(V/V) as mobile phase the flow rate was 0 7mL·min -1 and the wavelength of detection was at 250nm.RESULTS The calibration curve was showed good linearity within the range of 0 404～2 020μg,r=0 9999,the average recovery was 99 23%,RSD=1 29%;and the RSD value of presity was 0 26%.CONCLUSION The method can eliminate interference of other components and is accurate,precise and simple.

Key concepts: Puerarin, Chromatography, Calibration curve, High-performance liquid chromatography, Chemistry, Methanol, Stationary phase, Volumetric flow rate

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