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Cloning of 5′ Region of Goose Prolactin Receptor cDNA

Kong Ge-ping

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Abstract

To develop the genetic markers linked to broodiness,the 5′region of the goose prolactin receptor(gPRLR) gene from a matured male Hongzehu Goose testis tissue was defined using RACE.Compared with cPRLR gene from chicken,the cloned sequence(499 bp) is divided into a 348 bp 5′-UTR region and a 151 bp coding region with ATG start codon which were further divided into exon 1,2,3 and partial sequence of exon 4 with the length of 238 bp,69 bp,114 bp and 81 bp,respectively.The open reading frame starts from the 45st base in exon 3.The cloned coding region shares 88 % identity to the cPRLR cDNA sequence and 84% identity to deduced amino acid sequence.

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What this paper is about

To develop the genetic markers linked to broodiness,the 5′region of the goose prolactin receptor(gPRLR) gene from a matured male Hongzehu Goose testis tissue was defined using RACE.Compared with cPRLR gene from chicken,the cloned sequence(499 bp) is divided into a 348 bp 5′-UTR region and a 151 bp coding region with ATG start codon which were further divided into exon 1,2,3 and partial sequence of exon 4 with the length of 238 bp,69 bp,114 bp and 81 bp,respectively.The open reading frame starts from the 45st base in exon 3.The cloned coding region shares 88 % identity to the cPRLR cDNA sequence and 84% identity to deduced amino acid sequence.

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Available abstract

To develop the genetic markers linked to broodiness,the 5′region of the goose prolactin receptor(gPRLR) gene from a matured male Hongzehu Goose testis tissue was defined using RACE.Compared with cPRLR gene from chicken,the cloned sequence(499 bp) is divided into a 348 bp 5′-UTR region and a 151 bp coding region with ATG start codon which were further divided into exon 1,2,3 and partial sequence of exon 4 with the length of 238 bp,69 bp,114 bp and 81 bp,respectively.The open reading frame starts from the 45st base in exon 3.The cloned coding region shares 88 % identity to the cPRLR cDNA sequence and 84% identity to deduced amino acid sequence.

Key concepts: Goose, Exon, Coding region, Biology, Complementary DNA, Genetics, Prolactin receptor, Open reading frame

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