Cloning of 3′-end of Prolactin Receptor Gene in Goose

To characterize the prolactin receptor gene family of goose,a cDNA fragment of goose prolactin receptor gene(gPRLR) with a length of 606 bp was first isolated by RT-PCR using a pair of degenerate primers which were designed based on conservative regions among six species incluing of human,chicken,turkey,pigeon,pig and rat.The 3′-end of gPRLR cDNA was identified by 3′-RACE strategy using gene-specific primers complementary to the isolated cDNA fragment.Sequence analysis of the 1 876 bp RACE product showed a 1 656 bp 3′coding region,a stop codon(TAA) and a 220 bp 3′-UTR.Six predicted exons with sizes of 148 bp,170 bp,145 bp,100 bp, 70 bp and 1 243 bp,respectively,which were found in the sequences were highly homologous to the last exons of cPRLR cDNA.The C-terminus of gPRLR with 551 amino acids long encoded by the last 1 653 bp of the region exhibited 87.7% similarity to the corresponding region of chicken prolactin receptor.Stop codon was located at 1 021 bp of last exon.