Cloning and Sequence Analysis of Partial Fragment of Prolactin Receptor Gene in Goats

The 892 bp fragment(97 bp of part of exon 8(the full length of exon 8 was 100 bp),683 bp of intron 8,70 bp of exon 9 and 42 bp of part of intron 9)of prolactin receptor(PRLR) gene was amplified successfully in Jining Grey goats by PCR and cloned into pGEM-T Easy vector.The positive clones were further identified by PCR analysis.The nucleotide sequence was detected and the peptide sequence of this fragment was deduced.This sequence shared 99.4 %,97.01%,89.22%,89.22%,88.02% nucleotide homology with the published mRNA of PRLR gene of sheep,cow,human,rat and mouse separately,and the amino acid homology was 100%,94.55%,81.88%,81.82%,83.64% separately.