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Content determination of Imperatorin, Tetrahydropalmatine and Isoimperatorin in Yuanhu Zhitong Tablets by HPLC

LI Yanxue

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Abstract

Objective:To establish a method for the content determination of imperatorin, tetrahydropalmatine and isoimperatorin in Yuanhu Zhitong Tablets by HPLC. Methods:The Agilent ZORBAX SB-C18 (4.6 mm×250 mm, 5 μm) column was used. The mobile phases consisted of methanol and phosphatic liquor Solution(triethylamine ajusted to pH=6.5) (61 ∶39), the flow rate was 1.0 ml/min and the detection wavelength was at 280 nm. Results: The linear ranges of imperatorin was at 0.047 4-0.284 4 μg (r=0.999 9), the linear ranges of tetrahydropalmatine was at 0.020 5-0.123 0 μg (r=0.999 8), the linear ranges of isoimperatorin was at 0.075 0-0.450 0 μg (r=1.000 0), the average recoveries were 98.6%, 97.5% and 96.8%, respectively. Conclusion: The method is fast, reliable, accurate and can be used in the quality control of Yuanhu Zhitong Tablets.

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Objective:To establish a method for the content determination of imperatorin, tetrahydropalmatine and isoimperatorin in Yuanhu Zhitong Tablets by HPLC. Methods:The Agilent ZORBAX SB-C18 (4.6 mm×250 mm, 5 μm) column was used. The mobile phases consisted of methanol and phosphatic liquor Solution(triethylamine ajusted to pH=6.5) (61 ∶39), the flow rate was 1.0 ml/min and the detection wavelength was at 280 nm. Results: The linear ranges of imperatorin was at 0.047 4-0.284 4 μg (r=0.999 9), the linear ranges of tetrahydropalmatine was at 0.020 5-0.123 0 μg (r=0.999 8), the linear ranges of isoimperatorin was at 0.075 0-0.450 0 μg (r=1.000 0), the average recoveries were 98.6%, 97.5% and 96.8%, respectively. Conclusion: The method is fast, reliable, accurate and can be used in the quality control of Yuanhu Zhitong Tablets.

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Available abstract

Objective:To establish a method for the content determination of imperatorin, tetrahydropalmatine and isoimperatorin in Yuanhu Zhitong Tablets by HPLC. Methods:The Agilent ZORBAX SB-C18 (4.6 mm×250 mm, 5 μm) column was used. The mobile phases consisted of methanol and phosphatic liquor Solution(triethylamine ajusted to pH=6.5) (61 ∶39), the flow rate was 1.0 ml/min and the detection wavelength was at 280 nm. Results: The linear ranges of imperatorin was at 0.047 4-0.284 4 μg (r=0.999 9), the linear ranges of tetrahydropalmatine was at 0.020 5-0.123 0 μg (r=0.999 8), the linear ranges of isoimperatorin was at 0.075 0-0.450 0 μg (r=1.000 0), the average recoveries were 98.6%, 97.5% and 96.8%, respectively. Conclusion: The method is fast, reliable, accurate and can be used in the quality control of Yuanhu Zhitong Tablets.

Key concepts: Imperatorin, Tetrahydropalmatine, Chromatography, High-performance liquid chromatography, Triethylamine, Content determination, Medicine, Linear relationship

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