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2004•Strait Pharmaceutical JournalRequires access

Determination of Tetrahydropalmatine in Yuanhu Zhitong Tablets by HPLC

Zhitao Chen

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Abstract

OBJECTIVE To develop a method for determination of tetrahydropalmatine in Yuanhu Zhitong tablets.METHODS Using a HPLC method with Lichrospher ODS(4.6mm×250mm,5μm)column,the mobile phase was a mixture of 0.1% phosphoric acid-acetonitrile(77∶23),flow rate was 1.0ml·min -1 ,and column temperature was 25℃,with the detection wavelength at 281nm.RESULTS The precision and stability were fine,The linear correlation was observed for the concentration of 0.00992～0.04960mg·mL -1 (r=1.0000),The average rocovery of tetrahydropalmatine was 98.70% with RSD of 1.26%. The method was simple,accurate and reproducible,and can be used as a quality control method for Yuanhu Zhitong Teblets.

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What this paper is about

OBJECTIVE To develop a method for determination of tetrahydropalmatine in Yuanhu Zhitong tablets.METHODS Using a HPLC method with Lichrospher ODS(4.6mm×250mm,5μm)column,the mobile phase was a mixture of 0.1% phosphoric acid-acetonitrile(77∶23),flow rate was 1.0ml·min -1 ,and column temperature was 25℃,with the detection wavelength at 281nm.RESULTS The precision and stability were fine,The linear correlation was observed for the concentration of 0.00992～0.04960mg·mL -1 (r=1.0000),The average rocovery of tetrahydropalmatine was 98.70% with RSD of 1.26%. The method was simple,accurate and reproducible,and can be used as a quality control method for Yuanhu Zhitong Teblets.

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Available abstract

OBJECTIVE To develop a method for determination of tetrahydropalmatine in Yuanhu Zhitong tablets.METHODS Using a HPLC method with Lichrospher ODS(4.6mm×250mm,5μm)column,the mobile phase was a mixture of 0.1% phosphoric acid-acetonitrile(77∶23),flow rate was 1.0ml·min -1 ,and column temperature was 25℃,with the detection wavelength at 281nm.RESULTS The precision and stability were fine,The linear correlation was observed for the concentration of 0.00992～0.04960mg·mL -1 (r=1.0000),The average rocovery of tetrahydropalmatine was 98.70% with RSD of 1.26%. The method was simple,accurate and reproducible,and can be used as a quality control method for Yuanhu Zhitong Teblets.

Key concepts: Tetrahydropalmatine, Chromatography, Chemistry, Phosphoric acid, High-performance liquid chromatography, Content determination, Acetonitrile, Corydalis

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