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HPLC Determination of Imperatorin and Isoimperatorin in Yuanhu Zhitong Tablets

Xiaowei Wang

Open publisher page 2 citations

Abstract

Objective:To develop an HPLC method for the determination of imperatorin and isoimperatorin in Yua- nhu Zhitong tablets.Methods:C_(18)(4.6 mm×250 mm,5 μm)column was used.The mobile phase contained MeOH -H_2O(70:30).Flow rate was 0.8 mL·min~(-1).The UV detection wavelength was 254 nm.Results:The calibra- tion curve of imperatorin was linear(r=0.9999)in the range of 0.15-0.75μg,the mean recovery was 99.8%, with RSD 2.0%.The calibration curve of isoimperatorin was linear(r=0.9999)in the range of 0.10-0.50μg,the mean recovery was 98.1%,with RSD 2.4%.Conclusion:The method is simple,rapid and accurate.

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What this paper is about

Objective:To develop an HPLC method for the determination of imperatorin and isoimperatorin in Yua- nhu Zhitong tablets.Methods:C_(18)(4.6 mm×250 mm,5 μm)column was used.The mobile phase contained MeOH -H_2O(70:30).Flow rate was 0.8 mL·min~(-1).The UV detection wavelength was 254 nm.Results:The calibra- tion curve of imperatorin was linear(r=0.9999)in the range of 0.15-0.75μg,the mean recovery was 99.8%, with RSD 2.0%.The calibration curve of isoimperatorin was linear(r=0.9999)in the range of 0.10-0.50μg,the mean recovery was 98.1%,with RSD 2.4%.Conclusion:The method is simple,rapid and accurate.

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Available abstract

Objective:To develop an HPLC method for the determination of imperatorin and isoimperatorin in Yua- nhu Zhitong tablets.Methods:C_(18)(4.6 mm×250 mm,5 μm)column was used.The mobile phase contained MeOH -H_2O(70:30).Flow rate was 0.8 mL·min~(-1).The UV detection wavelength was 254 nm.Results:The calibra- tion curve of imperatorin was linear(r=0.9999)in the range of 0.15-0.75μg,the mean recovery was 99.8%, with RSD 2.0%.The calibration curve of isoimperatorin was linear(r=0.9999)in the range of 0.10-0.50μg,the mean recovery was 98.1%,with RSD 2.4%.Conclusion:The method is simple,rapid and accurate.

Key concepts: Imperatorin, Chemistry, Chromatography, Calibration curve, High-performance liquid chromatography, Linear range, Detection limit

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