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2009•Zhongguo yaofangRequires access

Determination of Three Active Components in Yuanhu Zhitong Tablet By HPLC

Guowei Zhao

Open publisher page 3 citations

Abstract

OBJECTIVE:To develop an HPLC method for simultaneous determination of tetrahydropalmatine, imperatorin and isoimperatorin in Yuanhu zhitong tablet. METHODS: The sample was separated on A Zorbax Extend-C18 column (250 mm×4.6 mm, 5 μm) at column temperature of 25 ℃. The mobile phase consisted of acetonitrile-0.1% phosphoric acid solution (adjusted pH to 6.5 by triethylamine, gradient elution) at a flow rate of 1 mL·min-1. RESULTS: The linear ranges of tetrahydropalmatine, imperatorin and isoimperatorin were 10.2～51.0 μg·mL-1(r=0.999 9), 2.5～12.5 μg·mL-1(r= 0.999 9) and 2.2～11.0 μg·mL-1(r=0.999 9) respectively, and their average recovery rates were 97.89%(RSD=0.8%, n=9), 101.77%(RSD=1.4%, n=9) and 99.04%(RSD=1.3%, n=9), respectively. CONCLUSION: The method is simple, rapid, accurate, and applicable for the quality control of Yuanhu zhitong tablet.

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What this paper is about

OBJECTIVE:To develop an HPLC method for simultaneous determination of tetrahydropalmatine, imperatorin and isoimperatorin in Yuanhu zhitong tablet. METHODS: The sample was separated on A Zorbax Extend-C18 column (250 mm×4.6 mm, 5 μm) at column temperature of 25 ℃. The mobile phase consisted of acetonitrile-0.1% phosphoric acid solution (adjusted pH to 6.5 by triethylamine, gradient elution) at a flow rate of 1 mL·min-1. RESULTS: The linear ranges of tetrahydropalmatine, imperatorin and isoimperatorin were 10.2～51.0 μg·mL-1(r=0.999 9), 2.5～12.5 μg·mL-1(r= 0.999 9) and 2.2～11.0 μg·mL-1(r=0.999 9) respectively, and their average recovery rates were 97.89%(RSD=0.8%, n=9), 101.77%(RSD=1.4%, n=9) and 99.04%(RSD=1.3%, n=9), respectively. CONCLUSION: The method is simple, rapid, accurate, and applicable for the quality control of Yuanhu zhitong tablet.

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Available abstract

OBJECTIVE:To develop an HPLC method for simultaneous determination of tetrahydropalmatine, imperatorin and isoimperatorin in Yuanhu zhitong tablet. METHODS: The sample was separated on A Zorbax Extend-C18 column (250 mm×4.6 mm, 5 μm) at column temperature of 25 ℃. The mobile phase consisted of acetonitrile-0.1% phosphoric acid solution (adjusted pH to 6.5 by triethylamine, gradient elution) at a flow rate of 1 mL·min-1. RESULTS: The linear ranges of tetrahydropalmatine, imperatorin and isoimperatorin were 10.2～51.0 μg·mL-1(r=0.999 9), 2.5～12.5 μg·mL-1(r= 0.999 9) and 2.2～11.0 μg·mL-1(r=0.999 9) respectively, and their average recovery rates were 97.89%(RSD=0.8%, n=9), 101.77%(RSD=1.4%, n=9) and 99.04%(RSD=1.3%, n=9), respectively. CONCLUSION: The method is simple, rapid, accurate, and applicable for the quality control of Yuanhu zhitong tablet.

Key concepts: Imperatorin, Tetrahydropalmatine, Chemistry, Chromatography, Triethylamine, Phosphoric acid, High-performance liquid chromatography, Gradient elution

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