Comparative Study on the Content of Paeonol and Paeoniflorin in Cortex Moutan from Tongling and Bozhou

Objective:To determine the content of Paeonol and Paeoniflorin in Cortex Moutan from Tongling and Bozhou,and compare the quality difference. Methods: chromatographic column: Waters VP-ODS-C18(150 mm ×4.6 mm, 5 μm); Mobile phase for paeonol: methanol-water(60:40), Detection wavelength: 274 nm, Column temperature: 30℃, the flow rate: 1.0 mL·min-1;Mobile phase for paeoniflorin:acetonitrile-0.1% H3PO4(20∶80), Detection wavelength: 230 nm, Column temperature: 30 ℃, the flow rate:1.0 mL·min-1. Results: Paeonol and paeoniflorin had good linear relationship in 1.073-5.365 μg(r=0.9999)and 0.593-2.965 μg(r=0.9991)respectively. The average recoveriey of paeonol was 99.96%(RSD =0.72%), and paeoniflorin was 99.03%(RSD =0.49%).Conclusion: The experiment show that the content of the Paeonol localed at the Tongling region Cortex Moutan is much more higher than the Bozhou region Cortex Moutan and the content of the Paeoniflorin localed at the Tongling region Cortex Moutan is slightly lower than the Bozhou region Cortex Moutan.