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2011•Traditional Chinese Drug Research and Clinical PharmacologyRequires access

Determination of Paeoniflorin and Paeonol in Cortex Moutan by RP-HPLC

Zhang Liuji

Open publisher page 2 citations

Abstract

Objective To establish a RP-HPLC method for determining paeoniflorin and paeonol in Cortex Moutan.Methods A SunFire ODS C18 column(4.6 mm × 250 mm,5 μm)was used,with the mobile phase consisting of methanol-0.1 % phosphoric acid in gradient mode,at the flow rate of 1.0mL / min.The UV detection wavelength was set at the maximum absorption wavelength,230 nm for paeoniflorin,and 274nm for paeonol.Results The linear ranges of paeoniflorin and paeonol were 0.212～2.120 μg(r=0.999 2) and 0.598～5.980 μg(r=0.999 8),respectively.The average recoveries of paeoniflorin and paeonol were 97.94 %(RSD =1.87 %) and 100.62 %(RSD =1.63 %),respectively.Conclusion This method is sample,accurate and repeatable.It can be used for the quality control of Cortex Moutan.

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Objective To establish a RP-HPLC method for determining paeoniflorin and paeonol in Cortex Moutan.Methods A SunFire ODS C18 column(4.6 mm × 250 mm,5 μm)was used,with the mobile phase consisting of methanol-0.1 % phosphoric acid in gradient mode,at the flow rate of 1.0mL / min.The UV detection wavelength was set at the maximum absorption wavelength,230 nm for paeoniflorin,and 274nm for paeonol.Results The linear ranges of paeoniflorin and paeonol were 0.212～2.120 μg(r=0.999 2) and 0.598～5.980 μg(r=0.999 8),respectively.The average recoveries of paeoniflorin and paeonol were 97.94 %(RSD =1.87 %) and 100.62 %(RSD =1.63 %),respectively.Conclusion This method is sample,accurate and repeatable.It can be used for the quality control of Cortex Moutan.

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Available abstract

Objective To establish a RP-HPLC method for determining paeoniflorin and paeonol in Cortex Moutan.Methods A SunFire ODS C18 column(4.6 mm × 250 mm,5 μm)was used,with the mobile phase consisting of methanol-0.1 % phosphoric acid in gradient mode,at the flow rate of 1.0mL / min.The UV detection wavelength was set at the maximum absorption wavelength,230 nm for paeoniflorin,and 274nm for paeonol.Results The linear ranges of paeoniflorin and paeonol were 0.212～2.120 μg(r=0.999 2) and 0.598～5.980 μg(r=0.999 8),respectively.The average recoveries of paeoniflorin and paeonol were 97.94 %(RSD =1.87 %) and 100.62 %(RSD =1.63 %),respectively.Conclusion This method is sample,accurate and repeatable.It can be used for the quality control of Cortex Moutan.

Key concepts: Paeonol, Paeoniflorin, Chromatography, Chemistry, Phosphoric acid, High-performance liquid chromatography, Medicine, Organic chemistry

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